Figure 5
From: Cucurbitacin B and I inhibits colon cancer growth by targeting the Notch signaling pathway
Cucurbitacin B and I downregulates Notch signaling pathway. (A) Cells lysates treated with C-B and C-I caused a significant reduction in the expression of first cleavage proteins ADAM9 and TACE in both HCT116 and SW480 cells. (B) Cells lysates treated with C-B and C-I caused a significant reduction in the expression of Notch receptors Notch-1, 2, 3 and 4 in both HCT116 and SW480 cells. (C) Cells lysates treated with C-B and C-I caused a significant reduction in the expression of Notch ligands Jagged-1, Jagged-2, DLL1, DLL3, and DLL4 in both HCT116 and SW480 cells. (D) Cells lysates treated with C-B and C-I caused a significant reduction in the expression of γ-secretase complex proteins nicastrin and presenilin1 in both HCT116 and SW480 cells. (E) Cells lysates treated with C-B and C-I caused significant reduction in the expression of Notch intracellular domain binding partners in the nucleus MAML1, HAT1 and RBPSUH in both HCT116 and SW480 cells. (F) Cells lysates treated with C-B and C-I caused significant reduction in the expression of Notch receptors target genes Cyclin D1, c-Myc and Hes-1 in both HCT116 and SW480 cells.
