Table 2 Quality control of the Rhinella arenarum transcriptome. Quality scores were calculated using TRANSRATE v1.0.3, BUSCO v3.0.2, and DETONATE v1.9 before and after the CD-HIT clustering tool.

From: The Rhinella arenarum transcriptome: de novo assembly, annotation and gene prediction

 

Before CD-HIT

After CD-HIT

TRANSRATE v1.0.3

Transrate Assembly Score

0.0457

0.158

Transrate Optimal Score

0.1172

0.2092

Transrate Optimal Cutoff

0.129

0.0928

good contigs

163674

173616

p good contigs

0.66

0.87

BUSCO v3.0.2

Complete BUSCOs (C)

973 (99.4%)

972 (99.4%)

Complete and single-copy BUSCOs (S)

507 (51.8%)

740 (75.7%)

Complete and duplicated BUSCOs (D)

466 (47.6%)

232 (23.7%)

Fragmented BUSCOs (F)

0 (0.0%)

0 (0.0%)

Missing BUSCOs (M)

5 (0.6%)

6 (0.6%)

Total BUSCO groups searched

978

978

DETONATE v1.9

Score

−14949658702

−15357930779

BIC_penalty

−2381204

−1893606,56

Prior_score_on_contig_lengths_(f_function_canceled)

−809045

−628519,94

Prior_score_on_contig_sequences

−339449528

−220853915,1

Data_likelihood_in_log_space_without_correction

−14607924788

−15135245313

Correction_term_(f_function_canceled)

−905861

−690575,74