Figure 6

Lung neutrophils during primary RSV infection do not affect memory T cell responses upon re-challenge. (a) C57BL/6 or wt mice were mock (PBS) or RSV infected. To deplete neutrophils, 150 µg i.p. α-Ly6G or isotype control antibody was administered on days −1, 1, 3, 5 and 7. Mice were re-challenged with RSV on day 21. (b) Total numbers of lung CD4⁺ (CD19⁻, CD3⁺), CD8⁺ (CD19⁻, CD3⁺) and CD8⁺ T_RM (CD19⁻, CD3⁺, CD8⁺, CD62L⁻, CD44⁺, CD69⁺, CD103⁺; Supp. Figure 1 for full gating strategy). (c) Frequency of GzmB⁺ and IFN-γ⁺ producing CD8⁺ T_RM cells. (d) Total number of airway CD8⁺ (CD19⁻, CD3⁺) T cells, and e. total number and frequency of airway CD8⁺ T_RM (CD19⁻, CD3⁺, CD8⁺, CD62L⁻, CD44⁺, CD69⁺, CD103⁺). Each symbol represents an individual mouse. Data from (b,d,e) are presented as mean ± SEM of 8–15 individual mice pooled from two or three independent experiments. Data from (c) are presented as mean ± SEM of 5–10 individual mice from one or pooled from two independent experiments. Statistical significance of differences was determined by one-way ANOVA with Tukey’s post hoc test. Only the statistical significances between RSV re-infected groups are shown. *P ≤ 0.05.