Figure 4
From: Hepatic ILC2 activity is regulated by liver inflammation-induced cytokines and effector CD4+ T cells
Antigen-specific cross-talk of hepatic ILC2 and CD4+ T cells. (a) C57BL/6 mice were treated with IL-33 on four consecutive days. Hepatic ILC2 from naive and IL-33-treated mice were stained for MHCII, CD80, and CD86 and analysed by flow cytometry. (b) Hepatic ILC2 from IL-33-treated mice were co-cultured with OVA-specific CD4+ T cells from OT-II mice in presence or absence of OVA for 4 days. Phenotype analysis was done in TCRβ+ CD4+ T cells and TCRβ- ILC2. (c) Cells were counted and expansion was calculated in comparison to the co-culture without OVA. (d) Activation and intracellular cytokine expression were analysed in CD4+ T cells and (e) hepatic ILC2 by flow cytometry. (f) Co-cultures of hepatic ILC2 and CD4+ T cells were done in presence or absence of OVA and an anti-IL-2 antibody for 4 days. Dot plot shows frequencies of CD4+ T cells and ILC2. Histograms show frequencies of cytokine-expressing hepatic ILC2 and CD4+ T cells. Bold line, antibody staining; filled graph, fluorescence minus one control. Mean ± SEM of 2–3 independent experiments are shown. (a,c,e) Mann-Whitney U test; (c,d,f) one-way ANOVA with post analysis by Tukey-Kramer test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns: not significant.
