Figure 4
From: Core gene-based molecular detection and identification of Acanthamoeba species

Phylogenetic tree for 18S ribosomal DNA of Acanthamoeba species. The genotypes based on previous studies or suggested here were represented for each species. Five hundred bootstrap replicates were performed. The scale bars indicate the number of nucleotide substitutions per site. In gray: 18S ribosomal DNA sequences retrieved from the NCBI GenBank nucleotide sequence database for reference strains; in black: 18S ribosomal DNA sequences obtained using primers designed in the present study; in pink: 18S ribosomal DNA sequences retrieved from the draft genome sequences of Acanthamoeba species.