Table 3 Set of primers designed for 18S ribosomal DNA from Acanthamoeba spp.
From: Core gene-based molecular detection and identification of Acanthamoeba species
Primer name | Forward/Reverse | Sequence 5′-3′ | Length (nucleotides) | Amplicon size (bp) |
|---|---|---|---|---|
Acant_18S_F1 | Forward | TCATATGCTTGTCTCAAAGAT | 21 | 700 |
Acant_18S_R1 | Reverse | GCTTTTTAACTGCAACAACTT | 21 | |
Acant_18S_F2 | Forward | GCGGTAATTCCAGCTCCAAT | 20 | 678 |
Acant_18S_R2 | Reverse | TGGTGTTTTGTATTCAACGTC | 21 | |
Acant_18S_F3 | Forward | ACCATAAACGATGCCGACCA | 20 | 700 |
Acant_18S_R3 | Reverse | ACTCGTTGGATTAATCAGTGT | 21 | |
Acant_18S_F4 | Forward | CCTTAGATGTTCTGGGCCG | 19 | 452 |
Acant_18S_R4 | Reverse | GACCTTGTTACGACTTCTCC | 20 |
