Figure 3 | Scientific Reports

Figure 3

From: The PoV mycovirus affects extracellular enzyme expression and fruiting body yield in the oyster mushroom, Pleurotus ostreatus

Figure 3

Relative transcript abundances of the PoV gene in P. ostreatus based on quantitative real-time polymerase chain reaction (qRT-PCR). Expression levels of the PoV gene in dsRNA extracts from mycelia (A) and fruiting bodies (B) of the virus-infected strain (POV) and the virus-cured strains (POVF10 and POVF12) were analyzed. In both assays, a housekeeping gene (actin) was used as an internal control. The black bar indicates analyses of the POV strain. The other two bars indicate analyses of the virus-cured POVF10 and POVF12 strains. To detect PoV, qRT-PCR was performed using three biological and technical replicates. (C) Results from qRT-PCR analysis of the PoV mycovirus in the mycelium (MPOV) and fruiting body (FPOV) of the POV strain. The actin gene was used as an internal control. The y-axis represents the expression levels of the genes relative to the actin gene in P. ostreatus. The black and light gray bars indicate analyses of the POV and POVF10 and POVF12 strains, respectively. A value of p < 0.05 denotes statistical significance.

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