Figure 4

Bavendamm assay of a mycovirus-infected P. ostreatus strain. (A) Colonies were grown for 5 days on tannic acid-containing medium. The intensity of brown coloration correlates to the polyphenol oxidase activity in each strain. Top and bottom views of the Bavendamm’s plates are shown. The numbers 1, 2, and 3 indicate the virus-infected POV strain, and virus-cured POVF10 and POVF12 strains, respectively. (B) The diameters of the fungal colonies of the POV, POVF10, and POVF12 strains. The colony diameter is measured using the length of the agar block. (C) The diameters of the brown zones produced by the POV, POVF10, and POVF12 strains. The black bar indicates analyses of the POV strain. The other two bars indicate analyses of the virus-cured POVF10 and POVF12 strains. Data are presented as the means ± standard deviations from four replicates and three independent experiments.