Table 1 Plasmids and strains used in this study.

From: An intra-bacterial activity for a T3SS effector

Plasmid

Source

FLAG-NleB (C. rodentium)

1

FLAG-NleB (C. rodentium) (DAD221–223/AAA)

1

GST-NleB (C. rodentium)

17

GST-NleB (C. rodentium) (DAD221–223/AAA)

17

GST-NleB1 (EHEC)

17

GST-NleB1 (EPEC)

17

GST-NleB2

17

GST-SseK1 (S. enterica)

17

GST-SseK2 (S. enterica)

17

GST-SseK3 (S. enterica)

17

His-FADD

17

His-GshA

This study

His-GshB

This study

His-GshB(R256A)

This study

FLAG-His-GshB

This study

FLAG-His-GshB(R256A)

This study

His-CesT

This study

His-GST

Novagen

Strain

Source

C. rodentium DBS100

44

C. rodentium DBS100 ΔnleB

1

C. rodentium DBS100 ΔnleB/pFLAG-CTC-nleB

1

C. rodentium DBS100 ΔnleB/pFLAG-CTC-nleB DAD221–223/AAA

1

C. rodentium DBS100 ΔcesT

45

C. rodentium ΔgshB

This study

C. rodentium ΔgshB/pFLAG-CTC gshB

This study

C. rodentium ΔgshB/pFLAG-CTC gshB(R256A)

This study

S. enterica

46

S. enterica ΔsseK1

46

S. enterica ΔsseK2

46

S. enterica ΔsseK3

46

S. enterica ΔsseK1ΔsseK2

46

S. enterica ΔsseK1ΔsseK3

46

S. enterica ΔsseK2ΔsseK3

46

S. enterica ΔsseK1ΔsseK2ΔsseK3

46

E. coli BL21(DE3) x pET28a-gshA

This study

E. coli BL21(DE3) x pET28a-gshB

This study

E. coli BL21(DE3) x pET28a-gshB(R256A)

This study

E. coli BL21(DE3) x pET42a-nleB

This study

E. coli BL21(DE3) x pET42a-nleB DAD221-223/AAA

This study

E. coli BL21(DE3) x pET42a-nleB1 (EHEC)

17

E. coli BL21(DE3) x pET42a-nleB1 (EPEC)

17

E. coli BL21(DE3) x pET42a-nleB2

17

E. coli BL21(DE3) x pET42a-sseK1

17

E. coli BL21(DE3) x pET42a-sseK2

17

E. coli BL21(DE3) x pET42a-sseK3

17

E. coli O111:NM R82F2

36