Figure 6

Confocal imaging of cell transplantation experiments. (A,B) Representative pseudo coloured optical sections obtained by confocal microscopy for immunofluorescence staining for GFP (green pseudocolour), α-SMA (cyan pseudocolour) and VWF (red pseudocolour) (A) and Griffonia simplicifolia lectin (G.s., red pseudolour), GFP (green pseudocolour) and α-SMA (cyan pseudocolour) (B) shows multiple vWF⁺ GFP⁺ cells (arrows) and VWF⁺ GFP⁺ αSMA⁺ cells (arrowhead), indicating EnMT, as well as G.s.⁺ GFP⁺ (arrows), G.s.⁺ GFP⁺ α-SMA⁺ (arrowheads) cells in and around the vascular lesions. GFP⁺ cells are derived from the transplanted EC clones at 21 days in hypoxia + EC clones. The findings of GFP⁺ cells in the lung vasculature 21 days after injection suggests engraftment of the EC clones. The images show the optical sections, orthogonal sections of Z-stacks and sum projection of the Z-stacks. Inserts in projection images show GFP⁺ EC or GFP⁺ EnMT cells in more detail. Nuclear staining with DAPI (blue pseudocolour), scale bar: 20 μm.