Figure 2

High affinity between LNCaPRFP and NSCs. To assemble a 3-D co-culture, LNCaPRFP cells in single cell suspension were added to a 14-day culture of NSC spheroids in Neurosphere Medium. LNCaPRFP cells adhered to neurospheres, on which LNCaPRFP survived and proliferated for the first two weeks, after which LNCaPRFP growth rate declined and debris of red fluorescent cells was seen at day 28 of the co-culture.