Figure 4
Detection of the role of LH in male rats injected with EE2. After EE2 injection for 7 days, rats were sacrificed under anesthesia. The Leydig cells were purified from the testes. (A) Representative result of RIA of the concentration of testosterone release. Cells (1 × 105 cells per tube) were treated with the serial dosages of hCG (0.005, 0.01 or 0.05 IU/ml) for 1 h. At the end of the incubation, culture media were collected for testosterone RIA (n = 5). (B) Quantification of plasma LH level. Blood samples were collected through the abdominal aortic artery. Plasmas were isolated, the concentration of LH and measured by ELISA (n = 6). (C) Representative result and (D) quantification of the Western blot for LHR in Leydig cells. Leydig cells (3 × 106 cells) were incubated in culture medium for 1 h, and then cells were harvested for performing immunoblot analysis of LHR, and normalized to α-tubulin (α-Tu) expression (n = 5). Data represent means ± SEM. *P < 0.05, **P < 0.01 as compared with the EE2-untreated group. +P < 0.05, ++P < 0.01 as compared with the vehicle corresponding group.
