Figure 3 | Scientific Reports

Figure 3

From: Sex differences in Tfh cell help to B cells contribute to sexual dimorphism in severity of rat collagen-induced arthritis

Figure 3

Sex differences in the expression of co-stimulatory molecules CD40 and CD40L (CD154) on lymphocytes from CIA rats. Cells were obtained from lymph nodes draining inflamed joints and nearby tissues (dLNs) recovered at the peak of the clinical severity of CIA. (a) Representative flow cytometry dot plots show CD40 staining of CD45RA+ cells from dLNs of male and female rats. The number indicates the percentage in the region. Scatter plots show the frequency of CD40+ cells among CD45RA+ cells and CD40 mean fluorescence intensity (MFI) ratio of CD40+ CD45RA+ cells from dLNs of male and female rats. (b) Representative flow cytometry dot plots show CD40L (CD154) staining of CD4+ cells from dLNs of male and female rats. The number indicates the percentage in the region. Scatter plots show the frequency of CD40L + cells among CD4+ cells and CD40L mean fluorescence intensity (MFI) ratio of CD40L+ CD4+ cells from dLNs of male and female rats. Data are presented as means ± SEM. Horizontal lines within scatter plots indicate mean values. n = 8 rats per group. *p < 0.05, **p < 0.01.

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