Figure 3

Protein levels associated with membrane damage, cytoskeletal integrity, inflammation and autophagy are increased in nerve damaged TA, while oxidative activity appears to be decreased. Rat TA was subjected to western blot anaylsis of proteins associated with muscle damage. (A) Dysferlin, AnnexinA2, Cyclophilin, MLIM and LC3B are significantly upregulated in nerve damaged TA, while NOS (pan) levels are substantially decreased with nerve damage. (B) Bands corresponding to the western blot quantification in (A) (n = 5). The cropped pictures displayed above show the entire width of the membrane and all samples included for several different proteins. In order to allow for probing of multiple proteins with distinct molecular weight simultaneously, the membranes where cut horizontally at the appropriate heights. All band intensities were normalized to total protein content of the gel as assessed via coomassie blue staining. (C) Bands corresponding to the western blot quantification in (A) (n = 6). These blots were added during a later stage of the manuscript. All band intensities were normalized to total protein content of the gel as assessed via stain-free technology. *p < 0.05, **p < 0.01, ***p < 0.001.