Figure 3

Effects of Rikkunshito treatment on renal inflammation in sham- and UUO-operated mice. (a) Representative images of F4/80 immunostaining in kidneys of sham- and UUO-operated mice fed a standard diet and a diet containing Rikkunshito. The positive areas for F4/80 are evident as brown dots in the sections. Original magnification, ×200 (upper panels), and ×400 (lower panels). Scale bar = 100 μm. (b) F4/80-positive cells and (c) % F4/80 positive area in kidneys of sham- and UUO-operated mice fed a standard diet and a diet containing Rikkunshito. Quantitative analysis of inflammation-related gene expression in kidney of sham- and UUO-operated mice fed a standard diet and a diet containing Rikkunshito. (d) Monocyte chemoattractant protein-1 (MCP-1) and (e) interleukin (IL)-1β. Values are expressed as mean ± SE (n = 6 per group). *P < 0.05, **P < 0.01 vs sham-operated group. Differences between sham and UUO mice within each diet and between CTL and RKT within each surgery were analysed by two-factor ANOVA with Bonferroni’s post-test. CTL, control standard diet; RKT, diet containing Rikkunshito; UUO, unilateral ureteral obstruction.