Figure 4 | Scientific Reports

Figure 4

From: Effects of Rikkunshito treatment on renal fibrosis/inflammation and body weight reduction in a unilateral ureteral obstruction model in mice

Figure 4

Effects of Rikkunshito treatment on renal level of SIRT1, mitochondrial function-related genes, GHSR, and gastric ghrelin levels in sham- and UUO-operated mice. Quantitative analysis of mRNA levels of (a) sirtuin 1 (SIRT1), (c) peroxisome proliferator-activated receptor-γ coactivator 1α (PGC-1α), (d) Bcl-2/adenovirus E1B interacting protein 3 (BNIP3) in kidneys, and (f) ghrelin in stomach of sham- and UUO-operated mice fed a standard diet and a diet containing Rikkunshito. Immunoblot analysis of (b) SIRT1 and (e) GHSR protein levels in kidneys of sham- and UUO-operated mice fed a standard diet and a diet containing Rikkunshito. Values are expressed as the mean ± SE (a–e, n = 6 in each group. f, n = 7–8 in each group). *P < 0.05, **P < 0.01 vs sham-operated group. †P < 0.05 vs CTL group. Differences between sham and UUO mice within each diet and between CTL and RKT within each surgery were analysed by two-factor ANOVA with Bonferroni’s post-test. CTL, control standard diet; RKT, diet containing Rikkunshito; UUO, unilateral ureteral obstruction.

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