Figure 5

Histological examination to confirm hyperthermia-induced tumor killing. (a) Adjacent tumor sections were stained for Prussian blue to detect iron, GFP to detect viable tumor cells, and TUNEL to detect apoptotic cells. Tumor slices were obtained from four cohorts of ferumoxytol/AMF combinations. Red asterisk is placed to a region of Prussian blue positive and TUNEL negative regions. Yellow asterisk is placed to a region of Prussian blue positive and TUNEL positive. (b) Zoom-in view of the slice of tumor from +/+ of ferumoxytol/AMF cohort. Green circle indicates the site of intratumorally injected ferumoxytol. Blue circle is placed on tumor stroma (GFP negative) that stained positive both for Prussian blue and TUNEL.