Figure 3 | Scientific Reports

Figure 3

From: Repeated intramuscular transplantations of hUCB-MSCs improves motor function and survival in the SOD1 G93A mice through activation of AMPK

Figure 3

Activation of AMPK by hUCB-MSCs regulated iNOS and protein synthesis in C2C12 cell myotubes. (A) C2C12 cell myotubes were treated with TGF-β1 for 24 hr and cocultured with or without hUCB-MSCs and the cell lysates were probed with the indicated antibody. (B) The bands of (A) were quantified, and the relative expression levels are shown in the graph. (C) NO concentration in myotubes treated as described in (A) was measured using the Griess assay. (D) Representative graph showed relative change in the mRNA expression of SIRT1 and PGC1-alpha. All data were represented as mean ± SEM for three independent experiments and were analyzed by one-way analysis. *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page