Figure 4
Subnuclear localization of GLS2. Confocal microscopy of HepG2 cells treated with PMA. (A) GLS2-Nuclear speckles. Double immunofluorescence labeling with anti-GLS2 antibodies (left, green) and anti-nuclear speckles antibodies (center, red); right, view of double labeled cells showing only a few overlapping spots between the two immunolabels. (B) GLS2-Nucleolus. Double immunofluorescence labeling with anti-GLS2 antibodies (left, green) and antibodies against fibrillarin specific for cell nucleoli (center, red); right, merge view of double labeled cells: the nucleolar marker did not overlap with the nuclear GLS2 mark. (C) GLS2-Nucleoplasm. Confocal microscopy of HepG2 cells treated with PMA after double fluorescence labeling with anti-GLS2 antibodies (left, green) and anti-JMJD5 histone demethylase antibodies, a marker specific for cell nucleoplasm (center, red). (D) Double immunofluorescence labeling and confocal microscopy of HepG2 cell employing anti-VAMP8 (green) and anti-GLS2 (red) antibodies: the merge panel shows a high degree of overlapping marks.
