Figure 1 | Scientific Reports

Figure 1

From: Endomembrane Protein Trafficking Regulated by a TvCyP2 Cyclophilin in the Protozoan Parasite, Trichomonas vaginalis

Figure 1

TvCyP2 as a Myb3-binding protein. TvCyP2 was identified as a Myb3-binding protein by two-hybrid library screening. In A, utilizing a pair-wise two-hybrid interaction assay, pBait-Myb3, pBait-Myb3-N, and pBait-Myb3-C were each paired with pTRG-c22 or pTRG-c102. The relative strength of the interaction as revealed by the formation of colonies in each assay (−, no colony formation; +, >30 colonies; ++, >100 colonies) is summarized in the right panel. In B, the sequence of TvCyP2 (A2DLL4) was aligned to those of TvCyP1 (A2DT06), ceCYP3 (P52011) in Caenorhabditis elegans, yCPR1 (P14832) in yeast, and hCyPA (P62937) in humans. The amino acids indicated by asterisks are involved in enzymatic proficiency and CsA binding. The Gly-Pro bond (closed circle) and conserved Cys (closed triangle) are also indicated.

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