Figure 2
The enzymatic proficiency of TvCyP2. In the enzymatic reaction, 0~4 nM of GST-TvCyP2 (A) and GST-TvCyP2(R75A) (B) were each reacted with a chromogenic substrate. The enzymatic reaction was measured at 1-s intervals over 10 min by monitoring the OD390 with a spectrophotometer. The logarithmic phase of the enzyme reaction is plotted against various concentrations of GST-TvCyP2, and the calculated kcat/KM value is listed below each panel The inhibitory effect of cyclosporine A (CsA) (0~80 nM) on the enzymatic reaction of 4 nM GST-TvCyP2 is depicted in C.
