Figure 4
Subcellular localization of TvCyP2 in Trichomonas vaginalis. In (A), cell lysates from T. vaginalis in normal growth medium were examined by Western blotting using the anti-TvCyP2 antibody. In (B,C), an IFA of T. vaginalis cells was performed using the rat anti-TvCyP2, rabbit anti-TvBip, and mouse anti-TvCyP1 antibodies as indicated. Cells with (B and C top panel) or without (C, bottom panel) detergent permeation were then reacted with fluorescence-conjugated secondary antibodies. Nuclei were stained with DAPI. Fluorescence signals were recorded under confocal microscopy and merged. Cell morphology was recorded by phase-contrast microscopy. Bars in the micrographs represent 5 μm. In D–H), subcellular localization of TvCyP2, TvCyP1 or Myb3 was examined by the immunoelectron microscopy. Thin sections were double-stained by the rat anti-TvCyP1 and rabbit anti-TvBip (D), rat anti-TvCyP1 and rabbit anti-PFO (E), rat anti-TvCyP2 and rabbit anti-TvBip (F), rat anti-TvCyP2 and rabbit anti-rabbit PFO (G), or rat anti-TvArf-1 and rabbit anti-PFO (H). After washing, thin sections were reacted with the anti-rat IgG conjugated with 18-mm gold particles (closed triangles) and the anti-rabbit IgG conjugated with 12-nm gold particles (opened triangles). N, nucleus; ER, endoplasmic reticulum; (G), Golgi complex; H, hydrogenosome.
