Figure 6
TvCyP2 and nuclear translocation of Myb3. The stably expressing plasmid, pFLP-ha-TvCyP2, that overexpresses HA-TvCyP2 in Trichomonas vaginalis is depicted in (A). A mutation was introduced to produce pFLP-ha-TvCyP2(R75A). In (B), transfected cells overexpressing HA-TvCyP2, HA-TvCyP1(R75A), and non-transfected controls were sequentially reacted with the rat anti-HA antibody paired with FITC-conjugated rat IgG. Nuclei were stained with DAPI. Fluorescence signals (FITC and DAPI) were recorded under confocal microscopy and merged. Cell morphology was recorded under phase-contrast microscopy. Bars in the micrographs represent 5 μm. In C, cell lysates (TL) from cells overexpressing HA-TvCyP2 or HA-TvCyP2(R75A), and control cells were fractionated into cytosolic (C) and nuclear (N) fractions for Western blotting using antibodies for detecting various proteins as indicated. The relative intensities of Myb3 versus H3K9-Ac or TvCyP1 in the nuclear or cytosolic samples are quantified as shown in the histograms at the bottom. *p < 0.05. Error bars represent the standard deviation (n = 3).
