Figure 7
TvCyP2 and the subcellular distribution of membrane-bound Myb3. Samples from total lysates (A), crude cellular fractions (B), and enriched organelle fractions (C) from control cells (T1) and cells overexpressing HA-TvCyP2, HA-TvCyP2(R75A), or HA-TvCyP1 as indicated on top of each panel were examined by Western blotting using various antibodies to detect selected proteins as indicated on the right side of each panel. Molecular weights are indicated on the left side of each panel. Relative intensities of the protein bands in Western blotting from three independent experiments are quantified as shown at the bottom of each panel. *p < 0.05, **p < 0.01. In (D), cells with or without detergent permeation were double-stained with anti-HA and anti-TvCyP1 antibodies followed by secondary antibodies conjugated with FITC and Cy3. Nuclei were stained with DAPI. Signal intensities of TvCyP1 in hydrogenosomes and on the plasma membrane are quantified as shown in the histograms at the bottom. *p < 0.05, **p < 0.01. Error bars represent the standard deviation (n = 3).
