Figure 9

Observations of vesicles fused with or budding from hydrogenosomes. Vesicles (V) fused with (A-a) or budding from (A-b) a hydrogenosome (Hd) were observed by TEM. In B, cells after cryofixation (B-a, B-b, B-c) or chemical fixation (B-d) were embedded in Spurr resin. Thin sections from control cells (B-a) or transgenic cells overexpressing HA-TvCyP2 (B-b), HA-TvCyP1 (B-c), or HA-Myb3 (B-d) were sequentially reacted with an anti-HA antibody and 12-nm gold particles (closed triangles) conjugated to IgG. The fusion of a vesicle with or budding of a vesicle from a hydrogenosome is indicated by an open triangle. Bars in the micrographs represent 100 nm.