Figure 6
From: Native aggregation is a common feature among triosephosphate isomerases of different species
Separation of the aggregates of TbTIM by ion exchange chromatography. A total of 4.7 mg of TbTIM were loaded onto a Source 15 column. (A) UV signal from the elution pattern of the chromatography (blue line) applying a 50–100 mM NaCl gradient, four peaks are observed (colour arrow heads). Insert: Melting temperature of the four fractions. (B) SDS-PAGE gel of the fractions from the elution. (B) CN-PAGE of the fractions from the elution. The hydrodynamic radii of the different TIM species are shown to the right.
