Figure 8
From: Native aggregation is a common feature among triosephosphate isomerases of different species
Induced amyloid fibrillar TbTIM aggregates by heme peroxidase–H2O2–NO2−. Freshly purified dimeric enzyme was incubated with heme peroxidase–H2O2–NO2− (A) CN-PAGE from different incubation times (in hours) with the oxidative/nitrative stress agent. Red arrow head: dimeric TbTIM, green arrow head: horseradish peroxidase band. The numbers signalled the incubation time. (B) Denaturing temperature (Tm) and SPYRO orange fluorescence at different incubation times. (C) TEM images of TbTIM incubated 24 h with the oxidative/nitrative stress agent. Large characteristic fibrillar structures (orange arrow heads) are observed. The black line represents 200 nm. (D) Fluorescence microscopy images from fibrillar amyloid TbTIM aggregates stained with Thioflavine T prepared by 48 h incubation with the oxidative/nitrative stress agent. The black line represents 100 nm.
