Table 2 Thermodynamic constants of Impα/NLS complexes interactions. Data obtained by ITC assays.

From: Comparative study of the interactions between fungal transcription factor nuclear localization sequences with mammalian and fungal importin-alpha

Complex

Stoichiometry

Kd (μM)

ΔH (kcal/mol)

ΔS (cal/mol/deg)

MmImpα/PAC3

1.06 ± 0.01

0.44 ± 0.05

6.63 ± 0.66

6.47

MmImpα/PAC3Δminor

No binding

MmImpα/PAC3Δmajor

No binding

NcImpα/PAC352

1.02 ± 0.01

0.39 ± 0.07

−12.17 ± 0.17

−12.20

MmImpα/NIT2

0.95 ± 0.01

0.56 ± 0.23

−0.44 ± 0.19

−0.02

0.95 ± 0.01

5.74 ± 0.99

−8.09 ± 0.29

−0.40

NcImpα/NIT251

1.00 ± 0.01

0.56 ± 0.32

−6.64 ± 0.27

0.24

1.00 ± 0.01

9.90 ± 1.10

−7.04 ± 0.20

4.58