Figure 4
From: MBSP1: a biosurfactant protein derived from a metagenomic library with activity in oil degradation
Bacterial culture of clone MBSP1 and empty vector pHis-parallel1 after IPTG induction and emulsification assay with different hydrocarbon sources. (A) Emulsion observed in culture containing MBSP1 after induction with IPTG; (B) Emulsification index (E24%) showing similarity between MBSP1 and positive control 1% SDS, using different hydrocarbon sources. Statistical difference was only observed in relation to diesel emulsification (*p < 0.05); (C) Supernatant containing MBSP1 treated with different concentrations of proteinase K (0.4, 2, 1, 4 mg/ml). In the control, no proteinase K was added, and emulsifying activity was maintained.
