Table 3 Sequence of oligonucleotides and parameters used for amplification of gene coding for ORF 3C6.

From: MBSP1: a biosurfactant protein derived from a metagenomic library with activity in oil degradation

Protein

Restriction Enzyme (Sequence 5′−3′)

Oligonucleotides

MT*

Fragment size

ORF3C6

Foward_BsaI GGTCTCCCATGAGTGATCAATATCT

62,9 °C

897

pb Reverse_HindIII AAGCTTTTAAGTCGAGTCCTGACCC

64,6 °C

  1. *MT = Melting Temperature.
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