Figure 3 | Scientific Reports

Figure 3

From: Porphyromonas gingivalis triggers the shedding of inflammatory endothelial microvesicles that act as autocrine effectors of endothelial dysfunction

Figure 3

EMVPg induce pro-inflammatory and pro-oxidative protein expression similar to P. gingivalis infection. (A) TNF-α secretion in supernatant by EC in response to P. gingivalis (Pg) (MOI:100) and EMVPg (5, 20 and 30 nM) for 24 h was measured by ELISA. (B) Intra-cellular protein expression of eNOS, P21, ICAM-1, CDK4, P53, VCAM, iNOS and SOD-1 in response to P. gingivalis (Pg) (MOI:100) and EMVPg (5, 20 and 30 nM) for 24 h was evaluated by Western Blot. All data were expressed as mean ± SD from 3 independent experiments and normalized against internal control β-actin. *p < 0.05 between cells infected or stimulated against control (unstimulated cells).

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