Figure 3
From: Islet-1 synergizes with Gcn5 to promote MSC differentiation into cardiomyocytes
Identification of the key enzymes involved in histone methylation and DNA methylation. (a) HMT and DNMT bands were detected by Western blot, and the enzymes assessed were G9A, Suv39h1, DNMT-1, and DNMT-3a. Images were cropped for clarity, and full-length blots/gels are presented in Supplementary Fig. 2. (b,c) Quantitative analysis of these enzymes. In the process of inducing differentiation, G9A expression decreased gradually, DNMT-3a expression increased, and the other enzymes were not obviously changed. (d,e) The binding levels of these enzymes in the GATA4/Nkx2.5 promoter were detected by ChIP-qPCR; a normal mouse IgG antibody used as a negative control. The input% showed that the main HMT and DNMT binding in GATA4/Nkx2.5 promoters was G9A and DNMT-1, respectively. *p < 0.05 compared with the blank group. The error bars represent the SD of three independent experiments.
