Figure 4
From: Islet-1 synergizes with Gcn5 to promote MSC differentiation into cardiomyocytes
The expression of HP1 and its binding level in the GATA4/Nkx2.5 promoters. (a) HP1α and HP1β bands detected by Western blot. Images were cropped for clarity, and full-length blots/gels are presented in Supplementary Fig. 3. (b) Quantitative analysis of HP1α and HP1β. In the process of inducing differentiation, HP1α and HP1β expression was not obviously changed. (c) ChIP-qPCR detection of the binding level of HP1α and HP1β in GATA4/Nkx2.5 promoters. A normal mouse IgG antibody was used as a negative control. The input% showed that both HP1-α and HP1-β could bind to GATA4/Nkx2.5 promoters, but their binding level changed in only the GATA4 promoter. *p < 0.05 compared with the blank group. The error bars represent the SD of three independent experiments.
