Figure 5
From: Islet-1 synergizes with Gcn5 to promote MSC differentiation into cardiomyocytes
During MSC differentiation into cardiomyocytes induced by Islet-1, the effects of Gcn5 inhibition on other key enzymes were assessed. (a) Western blot was used to detect the expression of the enzymes involved in regulating GATA4/Nkx2.5 after Gcn5 was inhibited by MB-3. The enzymes assessed were Gcn5, HDAC1, G9A, DNMT-1 and HP1. Images were cropped for clarity, and full-length blots/gels are presented in Supplementary Fig. 4. (b) Quantitative analysis of these enzymes. MB-3 inhibited Gcn5 expression in both the Control + MB-3 and LV-islet-1 + MB-3 groups but had no effect on the other enzymes. (c,d) ChIP-qPCR results of the binding levels of these enzymes in the GATA4/Nkx2.5 promoters after Gcn5 was inhibited by MB-3 during MSC differentiation. After islet-1 transfection and Gcn5 inhibition, the binding level of the other enzymes in the GATA4/Nkx2.5 promoters did not change compared with those in the blank, control and control + MB-3 promoters. *p < 0.05 compared with the blank group, #p < 0.05 compared with the LV-islet-1 group. The error bars represent the SD of three independent experiments.
