Figure 6
From: Islet-1 synergizes with Gcn5 to promote MSC differentiation into cardiomyocytes
During MSC differentiation into cardiomyocytes induced by islet-1, changes in the levels of H3K9 acetylation/methylation and DNA methylation in the GATA4 and Nkx2.5 promoters after Gcn5 inhibition were detected. (a) ChIP-qPCR was used to detect H3K9 acetylation and methylation levels in the GATA4 and Nkx2.5 promoters. The input% showed H3K9 acetylation and methylation levels in the LV-islet-1 + MB-3 group, in contrast to those in the LV-islet-1 group. *p < 0.05 compared with the blank group, #p < 0.05 compared with the LV-islet-1 group. The error bars represent the SD of three independent experiments. (b) MSP was used to detect the DNA methylation level in the GATA4 and Nkx2.5 promoters. Two pairs of primers were designed by Methprimer: one set of methylation-specific primers and one set of nonmethylation-specific primers. Images were cropped for clarity, and full-length blots/gels are presented in Supplementary Fig. 5. (c) Bisulfite sequencing analysis of the GATA4 promoter. Methylated CG (filled circles) and unmethylated CG (open circles) are indicated. The methylation rate of the GATA4 promoter was 88% in the LV-islet-1 group and 98% in the LV-islet-1 + MB-3 group, which were the same as those in the blank and control groups.
