Figure 7
From: Islet-1 synergizes with Gcn5 to promote MSC differentiation into cardiomyocytes
The effect of Gcn5 inhibition on MSC differentiation into cardiomyocytes induced by islet-1. (a) FQ-PCR detected the expression levels of GATA4 and Nkx2.5 after Gcn5 was inhibited. GATA4 and Nkx2.5 expression increased after islet-1 transfection, but in the LV-islet-1 + MB-3 group, their expression did not change compared with that in the blank and control groups. *p < 0.05 compared with the blank group, #p < 0.05 compared with the LV-islet-1 group. The error bars represent the SD of three independent experiments. (b) An immunofluorescence assay was used to detect the expression of cTnT and Cx43 (100×). The fluorescent dye of cTnT was CY3, that of Cx43 was FITC, and DAPI was used for nuclear staining. cTnT express in Cytoplasm, and only expressed in LV-islet-1 group; Cx43 express mainly in Membrane, and expressed much more in LV-islet-1 group than other groups. The block in the lower left corner of a single image is the local amplification (200×) of the corresponding positions.
