Figure 2
From: Active nuclear import of the deacetylase Sirtuin-2 is controlled by its C-terminus and importins
SIRT2 interacts with multiple importins. SIRT2-GFP or GFP alone where immunoprecipitated using GFP-Trap® agarose beads for 1 hr. Cell lysates (INPUT) and IP fractions were immunoblotted using antibodies against GFP and (A) KPNA2, IPO7, TNPO1 or, (B) IPO13 and CBP which served as controls. Images are representative of 3 independent experiments. Uncropped blots are presented in Supplementary S3.
