Figure 4

Apelin regulated the infiltration of inflammatory cells and the expressions of cytokines and growth factors after cutaneous I/R. (A) Quantification of the numbers of infiltrating MPO⁺ neutrophils, CD68⁺ macrophages and CD3⁺ T cells in I/R site at 0, 1, 4 and 7 days after I/R treated with or without apelin. Values were determined by counting cells in six random microscopic fields in n = 4–5 mice per groups. (B) mRNA levels of growth factors and cytokines in I/R site at 0, 1, 4 and 7 days after I/R treated with or without apelin. n = 4–5 mice in each group. The number of cells or mRNA levels in control mice were assigned values of 1. All values represent mean ± SEM. **P < 0.01, *P < 0.05.