Figure 2 | Scientific Reports

Figure 2

From: Hypoxia-inducible factor (HIF) prolyl hydroxylase inhibitors induce autophagy and have a protective effect in an in-vitro ischaemia model

Figure 2

Annexin-V and 7-AAD FACS analysis of PC12 cells treated with HIF-PHD inhibitors (100 µM) in normoxia (21% O2) for 24 hours. (A) Representative dot plots of Annexin-V/7-AAD FACS analysis of cells treated with the vehicle (1% DMSO) and the inhibitors. Cells in lower left quadrant represent viable cells, cells in lower right quadrant represent early apoptosis and cells in upper right quadrant represent late apoptosis/necrosis; (B) The group data (n = 3) representing % of viable, early and late apoptotic cells. There was no significant different in any of the treatments groups compared to 1% DMSO treatment. Data were expressed as mean ± S.D.

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