Figure 1 | Scientific Reports

Figure 1

From: A combined Far-FTIR, FTIR Spectromicroscopy, and DFT Study of the Effect of DNA Binding on the [4Fe4S] Cluster Site in EndoIII

Figure 1

Schematic cartoon showing (a) FTIR microscope equipped with focal plane array (FPA) detector; (b) The extraction of 4096 spectra from the EndoIII/ds-DNA sample with the help of FPA detector, within an area of 175 × 175 µm2, immobilized on a gold-coated glass mirror; (c) an array of spectra extracted from the scanned area of the sample showing a regular increase in the intensity of the peaks from outside to inside region of the sample film; (d) the binding between the EndoIII (2ABK)10 containing the [4Fe4S] cluster and ds-DNA; (e) formation of an EndoIII/ds-DNA complex (1P59)28, with the shortest binding distance of 1.4 nm between the [4Fe4S] cluster and DNA double helix (f) the binding between DNA and the protein, which is manifested by changes in the Fe–S bond lengths, marked (i) and (ii), corresponding to Fe–S (thiolate) and Fe–S (sulfide) bonds of the [4Fe4S] cluster, respectively.

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