Figure 3

Micro-FTIR spectroscopy of the EndoIII/ds-DNA complex (a) Image showing the distribution of the EndoIII/ds-DNA complex film immobilized on a gold-coated glass substrate. The area marked in red is selected for 3D imaging and extraction of spectra; (b) chemical image showing the distribution of the amide-I spectral band intensity with an inset showing a 3D image of the red-highlighted area in (a–c) spectra selected from two different regions A and B of the sample, located as shown in the inset; (d) Array of spectra extracted from several pixels of the image.