Figure 2
Functionalities of HeartBeat software. (a–c) Graphical user interface of the HeartBeat software for computer-assisted semi-automated analysis allowing convenient and rapid quality check. (a) Standard deviations of pixel values relating to segmented heart regions are displayed over time in the left panel, where heartbeats are indicated by red asterisks for each image sequence. (b) A control panel allows (1) to refine settings of segmentation and to choose alternative segments, (2) to re-play the current image sequence (visualized in c), (3) to reset all settings or to save heart rates to spreadsheets together with a picture of segmented image sequence. (c) Visualization of the image sequence with segmented heart regions displayed as numbered blue areas (detailed manual available together with software in Supplementary material online).
