Figure 3
Heartbeat detection of multiple embryos per well. (a,b) Scoring of heart rate with 1–6 and 10 embryos per well for medaka at 102 hpf (a) and zebrafish at 34 hpf (b). Data is provided as box plots and scatter plots of original heart rate measurements. Heart rates of mounting groups with ≥2 embryos per well were each tested with Student’s t-test against 1 embryo per well (dashed line indicates mean heart rate of the group with 1 embryo per well); significant differences are indicated with *P < 0.05, **P < 0.01 and ***P < 0.001, ns (not significant); n for each group is indicated in the figure.
