Figure 3

QFAE adjusts AMPK signaling pathways in RAW 264.7 cells. (A) Protein expressions of total and phosphorylated AMPKα and ACC in LPS-induced RAW 264.7 cells were measured by Western blot, and α-Tubulin was used as a loading control. (B,C) Relative protein expressions of p-AMPKα/AMPKα and p-ACC/ACC in LPS-induced RAW 264.7 cells. (D) Protein expressions of total and phosphorylated AMPKα and ACC in none-induced RAW 264.7 cells were measured by Western blot, and α-Tubulin was used as a loading control. (E,F) Relative protein expressions of p-AMPKα/AMPKα and p-ACC/ACC in none-induced RAW 264.7 cells. Data are presented as the means ± SEM, n = 5. AP < 0.05 compared with model control group; BP < 0.05 compared with blank control group.