Figure 4 | Scientific Reports

Figure 4

From: A novel selection strategy for antibody producing hybridoma cells based on a new transgenic fusion cell line

Figure 4

Detection of the HA-tag on the cell surface of transfected myeloma cells. Stable transfected and non-transfected myeloma cells (1 × 106) were stained with a monoclonal anti-HA.11 antibody and a PE-labeled F(ab)2 fragment of a donkey anti-mouse IgG. After staining and washing the cells were analyzed by flow cytometry. Non-stained cells served as negative control. To exclude non-living cells a staining with 7-AAD was performed. The histograms represent all living cells from this approach with 1 × 105 recorded events. Analysis was performed by using BD CellQuest Pro software version 6.0.

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