Figure 2
TAZ mediates TNF-α-increased the proportion of breast cancer stem-like cell. (A) TAZ depletion blocks TNF-α-promoted BCSC increase, as measured by mammosphere culture. MCF7 cells were transfected with 20 nM TAZ siRNA for 48 h and then exposed to 10 ng/ml TNF-α or 0.1% BSA for 48 h. (B) Quantitative data for panel A. **P < 0.01, t-test. NS indicates significant. (C) TAZ protein levels were knocked down using siTAZ1# and siTAZ3#. Protein expression was determined by WB. (D) TAZ depletion blocks TNF-α-promoted BCSC increase, as measured by ALDH assays. MCF7 cells were transfected with 20 nM TAZ siRNA for 48 h and then exposed to 10 ng/ml TNF-α or 0.1% BSA for 48 h. Cells were collected for ALDH assays by FACS. The percentage of ALDH+ cells was quantified (mean ± SEM; n = 3). *P < 0.05, t-test. NS indicates not significant.
