Figure 2

Phylogenetic trees of MLB1 human astroviruses based on amplicon sequences of the capsid coding gene (357 bp) (A) and the RNA polymerase gene (370 bp) (B). Trees were constructed by Neighbor-Joining method with 500 bootstrap replicates. The evolutionary distances were computed using the p-distance method and are in the units of the number of base differences per site. Reference sequences for MLB1 and MLB2 HAstVs are denoted with a black color; sequences from control children are indicated in green, and sequences from children with gastroenteritis are denoted in red. Sequences obtained in the study were deposited in GenBank (accession numbers MN689595-MN689606).