Figure 5

The gram-scale production of (R)-3a via the kinetic resolution of 800 mM rac-3a using 200 mg wet cells/mL of E. coli/pveh3E134K/T137P. (a) The progress curves of the kinetic resolution of rac-3a within 8.0 h. (b) The chiral HPLC traces of the initial rac-3a (black line) as well as the retained (R)-3a and produced diols (red line) after rac-3a was incubated at 20 °C for 7.0 h.