Table 2 The EH activities and E values of six E. coli transformants towards rac-3a.

From: Significant improvement in catalytic activity and enantioselectivity of a Phaseolus vulgaris epoxide hydrolase, PvEH3, towards ortho-cresyl glycidyl ether based on the semi-rational design

E. coli transformant

Activity (U/g wet cell)

E value

E. coli/pveh3

6.0 ± 0.2

13.3 ± 0.7

/pveh3L128F

7.1 ± 0.3

15.5 ± 0.8

/pveh3L129M

7.0 ± 0.2

10.8 ± 0.5

/pveh3E134K

15.3 ± 0.4

21.7 ± 1.0

/pveh3T137P

16.1 ± 0.5

21.2 ± 1.1

/pveh3E134K/T137P

22.4 ± 0.6

24.1 ± 1.2

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