Table 3 The kinetic parameters of the purified PvEH3 and PvEH3E134K/T137P for (R)- and (S)-3a.

From: Significant improvement in catalytic activity and enantioselectivity of a Phaseolus vulgaris epoxide hydrolase, PvEH3, towards ortho-cresyl glycidyl ether based on the semi-rational design

Enzyme

(R)-3a

(S)-3a

kcatR (s−1)

KmR (mM)

kcatR/KmR (mM−1 s−1)

kcatS (s−1)

KmS (mM)

kcatS/KmS (mM−1 s−1)

PvEH3

1.12 ± 0.05

9.84 ± 0.28

0.11

9.82 ± 0.26

6.14 ± 0.22

1.60

PvEH3E134K/T137P

1.37 ± 0.06

5.40 ± 0.21

0.25

18.67 ± 0.52

3.29 ± 0.15

5.67

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